HPLC requires all mobile phases and samples to be as clean as possible during the analysis process. Otherwise, it will greatly damage the chromatographic instrument or column. In particular, the mobile phase, despite the user’s treatment by methods such as filtration before use, often brings in some mechanical impurities during use, which directly affects the life of the column and the analytical results.
Placed between the pump and sample injection valve protects valve seal wear and column from particles released through normal piston seal wear.
The HPLC inline filter impurities and solid particles from the mobile phase and protects the liquid phase system. It is usually installed at the back end of the pump, in front of the autosampler.
It is a ferrule type, but it is just with an HPLC frit, independent of the type of lc column. In practice, the installation position of the inline filter and the fact that it is only a frit to filter impurities from the mobile phase.
The HPLC inline filter can be used with any model of HPLC instrument for convenience and practicality. Because the connection holes of inline filters generally adopt the international standard for HPLC, i.e., 3/16in-32 thread, they can be easily connected to any pipeline needed for liquid chromatography. A common inline filter structure is shown in the figure.
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